Cell Senescence Entries for TFAP4

Cell Types
Retinal pigment epithelial, Breast cancer
Cell Lines
hTERT-RPE, MCF-7
Cancer Cell?
Yes
Method
Overexpression
Type of senescence
Replicative
Senescence Effect
Promotes
Observations
Long term cultures of confluent retinal pigment epithelial (RPE) cells were used as a model to examine the role of overexpression of AP4 (TFAP4), a highly expressed transcription factor in many types of cancer, in senescence. Stable inducible RPE cell clones expressing AP4 or AP4 without the DNA binding domain (DN-AP4) were cultured for 24 days. AP4-expressing cells exhibited large, flattened morphology, ceased proliferating, exhibited increased SA-beta-galactosidase activity and the SASP. These changes were not observed in DN-AP4-expressing cells or non-induced cells.
Primary Reference

    TFAP4 Gene Information

    HGNC symbol
    TFAP4 
    Aliases
    AP-4; bHLHc41 
    Common name
    transcription factor AP-4 
    Entrez Id
    7023
    Description
    Transcription factors of the basic helix-loop-helix-zipper (bHLH-ZIP) family contain a basic domain, which is used for DNA binding, and HLH and ZIP domains, which are used for oligomerization. Transcription factor AP4 activates both viral and cellular genes by binding to the symmetrical DNA sequence CAGCTG (Mermod et al., 1988 [PubMed 2833704]; Hu et al., 1990 [PubMed 2123466]).[supplied by OMIM, Jul 2009]

    TFAP4 Ontologies

    Gene Ontology
    Process: GO:6978; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator
    GO:71549; cellular response to dexamethasone stimulus
    GO:43922; negative regulation by host of viral transcription
    GO:43392; negative regulation of DNA binding
    GO:71157; negative regulation of cell cycle arrest
    GO:8285; negative regulation of cell proliferation
    GO:45736; negative regulation of cyclin-dependent protein serine/threonine kinase activity
    GO:45892; negative regulation of transcription, DNA-templated
    GO:43923; positive regulation by host of viral transcription
    GO:43065; positive regulation of apoptotic process
    GO:2001269; positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway
    And 6 more GO terms
    Cellular component: GO:5739; mitochondrion
    GO:5634; nucleus
    GO:17053; transcriptional repressor complex
    Function: GO:3677; DNA binding
    GO:70888; E-box binding
    GO:978; RNA polymerase II core promoter proximal region sequence-specific DNA binding
    GO:42826; histone deacetylase binding
    GO:5515; protein binding
    GO:46982; protein heterodimerization activity
    GO:42803; protein homodimerization activity
    GO:43565; sequence-specific DNA binding
    GO:3713; transcription coactivator activity
    GO:3705; transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
    GO:44212; transcription regulatory region DNA binding
    And 2 more GO terms
    Show all GO terms

    Homologs of TFAP4 in Model Organisms

    Caenorhabditis elegans
    hlh-11
    Danio rerio
    tfap4
    Drosophila melanogaster
    crp
    Mus musculus
    Tfap4
    Rattus norvegicus
    Tfap4

    External links

    OMIM
    600743
    Ensembl
    ENSG00000090447
    Entrez Gene
    7023
    UniGene
    513305
    1000 Genomes
    1000 Genomes
    HPRD
    GenAtlas
    TFAP4
    GeneCards
    TFAP4