Cell Senescence Entries for RAD21

Cell Types
Breast cancer
Cell Lines
MDA-MB-231
Cancer Cell?
No
Method
Knockdown
Type of senescence
Replicative
Senescence Effect
Inhibits
Observations
RAD21 knockdown resulted in the appearance of several senescent markers, including increased SA-beta-galactosidase activity and SA-heterochromatin foci formation, as well as elevated p21 protein levels and RB1 pathway activation. Further biochemical analysis revealed that RAD21 knockdown led to the downregulation of c-Myc and its targets including CDK4, a negative regulator of RB1. This blocked RB1 phosphorylation and the RB1-mediated transcriptional repression of E2F.
Primary Reference

    RAD21 Gene Information

    HGNC symbol
    RAD21 
    Aliases
    HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21 
    Common name
    RAD21 cohesin complex component 
    Entrez Id
    5885
    Description
    The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells. [provided by RefSeq, Jul 2008]

    RAD21 Ontologies

    Gene Ontology
    Process: GO:6310; DNA recombination
    GO:6915; apoptotic process
    GO:51301; cell division
    GO:6302; double-strand break repair
    GO:10972; negative regulation of G2/M transition of mitotic cell cycle
    GO:45841; negative regulation of mitotic metaphase/anaphase transition
    GO:45876; positive regulation of sister chromatid cohesion
    GO:45944; positive regulation of transcription from RNA polymerase II promoter
    GO:71168; protein localization to chromatin
    GO:7131; reciprocal meiotic recombination
    GO:6357; regulation of transcription from RNA polymerase II promoter
    And 3 more GO terms
    Cellular component: GO:785; chromatin
    GO:5694; chromosome
    GO:775; chromosome, centromeric region
    GO:8278; cohesin complex
    GO:5829; cytosol
    GO:16020; membrane
    GO:798; nuclear cohesin complex
    GO:5654; nucleoplasm
    Function: GO:3682; chromatin binding
    GO:5515; protein binding
    GO:32403; protein complex binding
    GO:1228; transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
    Show all GO terms

    Homologs of RAD21 in Model Organisms

    Danio rerio
    rad21a
    Drosophila melanogaster
    vtd
    Mus musculus
    Rad21
    Rattus norvegicus
    Rad21

    External links

    OMIM
    606462
    Ensembl
    ENSG00000164754
    Entrez Gene
    5885
    UniGene
    81848
    1000 Genomes
    1000 Genomes
    HPRD
    GenAtlas
    RAD21
    GeneCards
    RAD21