Cell Senescence Entries for PSMD14

Cell Types
Colorectal cancer, Melanoma, Pancreatic cancer, Lung cancer, Breast cancer, Cervical cancer, Prostate cancer, Ovarian cancer, Glioma
Cell Lines
DU145, NCI-H460, HCT116, HeLa, Mia PaCa-2, SNB-75, NCI-H1299, NCI-H23, NCI-H522, SK-LU-1, A427, A549, SK-BR-3, SK-MEL-2, HCC1937, BTS49-SK-OV-3
Cancer Cell?
Yes
Method
Knockdown
Type of senescence
Replicative
Senescence Effect
Inhibits
Observations
In several carcinoma cell types, down-regulation of PSMD14 by siRNA reduced cell viability causing cell arrest in the G0-G1 phase and ultimately leading to cellular senescence. This was accompanied by down-regulation of cyclin B1-CDK1-CDC25C, down-regulation of cyclin D1, up-regulation of p21 and p27, and reduction in phosphorylation of the Rb protein.
Primary Reference

    PSMD14 Gene Information

    HGNC symbol
    PSMD14 
    Aliases
    PAD1; POH1; RPN11 
    Common name
    proteasome 26S subunit, non-ATPase 14 
    Entrez Id
    10213
    Description
    This gene encodes a component of the 26S proteasome. The 26S proteasome is a large multiprotein complex that catalyzes the degradation of ubiquitinated intracellular proteins. The encoded protein is a component of the 19S regulatory cap complex of the 26S proteasome and mediates substrate deubiquitination. A pseudogene of this gene is also located on the long arm of chromosome 2. [provided by RefSeq, Feb 2012]

    PSMD14 Ontologies

    Gene Ontology
    Process: GO:38095; Fc-epsilon receptor signaling pathway
    GO:165; MAPK cascade
    GO:38061; NIK/NF-kappaB signaling
    GO:31146; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
    GO:50852; T cell receptor signaling pathway
    GO:60071; Wnt signaling pathway, planar cell polarity pathway
    GO:31145; anaphase-promoting complex-dependent catabolic process
    GO:2479; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
    GO:724; double-strand break repair via homologous recombination
    GO:6303; double-strand break repair via nonhomologous end joining
    GO:10972; negative regulation of G2/M transition of mitotic cell cycle
    And 20 more GO terms
    Cellular component: GO:5829; cytosol
    GO:31597; cytosolic proteasome complex
    GO:70062; extracellular exosome
    GO:5576; extracellular region
    GO:1904813; ficolin-1-rich granule lumen
    GO:5654; nucleoplasm
    GO:5634; nucleus
    GO:22624; proteasome accessory complex
    GO:502; proteasome complex
    GO:8541; proteasome regulatory particle, lid subcomplex
    GO:34774; secretory granule lumen
    And 1 more GO terms
    Function: GO:61578; Lys63-specific deubiquitinase activity
    GO:61133; endopeptidase activator activity
    GO:46872; metal ion binding
    GO:8237; metallopeptidase activity
    GO:70628; proteasome binding
    GO:5515; protein binding
    Show all GO terms

    Homologs of PSMD14 in Model Organisms

    Caenorhabditis elegans
    rpn-11
    Danio rerio
    psmd14
    Drosophila melanogaster
    Rpn11
    Mus musculus
    Psmd14
    Rattus norvegicus
    Psmd14
    Saccharomyces cerevisiae
    RPN11
    Schizosaccharomyces pombe
    rpn11

    In other databases

    GenAge model organism genes
    • A homolog of this gene for Caenorhabditis elegans is present as rpn-11

    External links

    OMIM
    607173
    Ensembl
    ENSG00000115233
    Entrez Gene
    10213
    UniGene
    740477
    1000 Genomes
    1000 Genomes
    HPRD
    GenAtlas
    PSMD14
    GeneCards
    PSMD14