Cell Senescence Entries for PNPT1

Cell Types
Melanoma
Cell Lines
HO-1, FO-1, MeWo
Cancer Cell?
Yes
Method
Overexpression
Type of senescence
Replicative
Senescence Effect
Promotes
Observations
Overexpression of PNPT1 in melanoma cells (HO-1, FO-1, MeWo) promoted a senescence-like phenotype characterized by growth arrest in the G1 phase, inhibition of DNA synthesis and telomerase activity, downregulation of c-myc and upregulation of senescence associated p27.
Primary Reference

    PNPT1 Gene Information

    HGNC symbol
    PNPT1 
    Aliases
    OLD35; DFNB70; PNPASE; old-35; COXPD13 
    Common name
    polyribonucleotide nucleotidyltransferase 1 
    Entrez Id
    87178
    Description
    The protein encoded by this gene belongs to the evolutionary conserved polynucleotide phosphorylase family comprised of phosphate dependent 3'-to-5' exoribonucleases implicated in RNA processing and degradation. This enzyme is predominantly localized in the mitochondrial intermembrane space and is involved in import of RNA to mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency-13 and autosomal recessive nonsyndromic deafness-70. Related pseudogenes are found on chromosomes 3 and 7. [provided by RefSeq, Dec 2012]

    PNPT1 Ontologies

    Gene Ontology
    Process: GO:6401; RNA catabolic process
    GO:35927; RNA import into mitochondrion
    GO:90503; RNA phosphodiester bond hydrolysis, exonucleolytic
    GO:43631; RNA polyadenylation
    GO:35458; cellular response to interferon-beta
    GO:34599; cellular response to oxidative stress
    GO:97421; liver regeneration
    GO:6402; mRNA catabolic process
    GO:965; mitochondrial RNA 3'-end processing
    GO:964; mitochondrial RNA 5'-end processing
    GO:957; mitochondrial RNA catabolic process
    And 17 more GO terms
    Cellular component: GO:5737; cytoplasm
    GO:5829; cytosol
    GO:5789; endoplasmic reticulum membrane
    GO:45025; mitochondrial degradosome
    GO:5758; mitochondrial intermembrane space
    GO:5739; mitochondrion
    GO:5634; nucleus
    GO:42788; polysomal ribosome
    Function: GO:175; 3'-5'-exoribonuclease activity
    GO:3723; RNA binding
    GO:35198; miRNA binding
    GO:34046; poly(G) binding
    GO:8266; poly(U) RNA binding
    GO:4654; polyribonucleotide nucleotidyltransferase activity
    GO:5515; protein binding
    Show all GO terms

    Homologs of PNPT1 in Model Organisms

    Caenorhabditis elegans
    BE0003N10.1
    Drosophila melanogaster
    CG11337-RD
    Mus musculus
    Pnpt1
    Rattus norvegicus
    Pnpt1

    External links

    OMIM
    610316
    Ensembl
    ENSG00000138035
    Entrez Gene
    87178
    UniGene
    388733
    1000 Genomes
    1000 Genomes
    HPRD
    GenAtlas
    PNPT1
    GeneCards
    PNPT1