Cell Senescence Entries for NTN4

Cell Types
Glioma
Cell Lines
U-251MG, U-U118MG, T98G, U-87MG
Cancer Cell?
Yes
Method
Overexpression, Knockdown
Type of senescence
Stress-induced
Senescence Effect
Inhibits
Observations
The suppression of either ITGB4 or NTN4 in glioblastoma cells (U87MG) significantly enhanced cellular senescence. The sensitivity of glioblastoma cells to TMZ was primarily determined by the expression of MGMT. To omit the effect of MGMT, cell lines devoid of MGMT expression were used. NTN4 partially inhibited TMZ induced cell senescence and rescued AKT from dephosphorylation in U251MG cells, a cell line bearing levels of ITGB4. However, addition of exogenous NTN4 displayed no significant effect on TMZ induced senescence rescue or AKT activation in U87MG cells, which expressed ITGB4 at low levels. Overexpression of ITGB4 combined with exogenous NTN4 significantly attenuated U87MG cell senescence induced by TMZ.
Primary Reference

    NTN4 Gene Information

    HGNC symbol
    NTN4 
    Aliases
    PRO3091 
    Common name
    netrin 4 
    Entrez Id
    59277
    Description
    This gene encodes a member of the netrin family of proteins, which function in various biological processes including axon guidance, tumorogenesis, and angiogenesis. Netrins are laminin-related proteins that have an N-terminal laminin-type domain, epidermal growth factor-like repeat domain, and a positively charged heparin-binding domain at the C-terminus. The protein encoded by this gene is involved in processes including neurite growth and migration, angiogenesis and mural cell adhesion to endothelial cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016]

    NTN4 Ontologies

    Gene Ontology
    Process: GO:7411; axon guidance
    GO:16322; neuron remodeling
    GO:60668; regulation of branching involved in salivary gland morphogenesis by extracellular matrix-epithelial cell signaling
    Cellular component: GO:5604; basement membrane
    GO:5886; plasma membrane
    Function: GO:43237; laminin-1 binding
    GO:5515; protein binding
    Show all GO terms

    Homologs of NTN4 in Model Organisms

    Danio rerio
    BX005001.1
    Mus musculus
    Ntn4
    Rattus norvegicus
    Ntn4

    External links

    OMIM
    610401
    Ensembl
    ENSG00000074527
    Entrez Gene
    59277
    UniGene
    201034
    1000 Genomes
    1000 Genomes
    HPRD
    GenAtlas
    NTN4
    GeneCards
    NTN4