Cell Senescence Entries for NOX4

Cell Types
Lung fibroblast, Breast epithelial
Cell Lines
MCF-12A, TIG-3
Cancer Cell?
No
Method
Overexpression, Knockdown
Type of senescence
Replicative
Senescence Effect
Promotes
Observations
Stable overexpression of NOX4 in normal breast epithelial cells resulted in H2O2 production, cellular senescence, resistance to apoptosis, and tumorigenic transformation. In contrast, inhibition of Nox4 in lung fibroblasts by RNA interference blocked the RasV12 senescent phenotype including beta-galactosidase activity, growth arrest and accumulation of tumor suppressors such as p53 and p16. This suggests that Nox-generated ROS transduce senescence signals by activating the p53 and p16 pathways.
Primary Reference
    Cell Types
    Lung fibroblast, Breast epithelial
    Cell Lines
    MCF-12A, TIG-3
    Cancer Cell?
    No
    Method
    Overexpression, Knockdown
    Type of senescence
    Replicative
    Senescence Effect
    Promotes
    Observations
    Stable overexpression of NOX4 in normal breast epithelial cells resulted in H2O2 production, cellular senescence, resistance to apoptosis, and tumorigenic transformation. In contrast, inhibition of Nox4 in lung fibroblasts by RNA interference blocked the RasV12 senescent phenotype including beta-galactosidase activity, growth arrest and accumulation of tumor suppressors such as p53 and p16. This suggests that Nox-generated ROS transduce senescence signals by activating the p53 and p16 pathways.
    Primary Reference

      NOX4 Gene Information

      HGNC symbol
      NOX4 
      Aliases
      KOX; KOX-1; RENOX 
      Common name
      NADPH oxidase 4 
      Entrez Id
      50507
      Description
      This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]

      NOX4 Ontologies

      Gene Ontology
      Process: GO:45453; bone resorption
      GO:55007; cardiac muscle cell differentiation
      GO:7569; cell aging
      GO:902; cell morphogenesis
      GO:71320; cellular response to cAMP
      GO:71480; cellular response to gamma radiation
      GO:71333; cellular response to glucose stimulus
      GO:71560; cellular response to transforming growth factor beta stimulus
      GO:10467; gene expression
      GO:50667; homocysteine metabolic process
      GO:6954; inflammatory response
      And 16 more GO terms
      Cellular component: GO:43020; NADPH oxidase complex
      GO:16324; apical plasma membrane
      GO:5789; endoplasmic reticulum membrane
      GO:5925; focal adhesion
      GO:16021; integral component of membrane
      GO:5739; mitochondrion
      GO:5730; nucleolus
      GO:5634; nucleus
      GO:48471; perinuclear region of cytoplasm
      GO:1725; stress fiber
      Function: GO:16174; NAD(P)H oxidase activity
      GO:9055; electron carrier activity
      GO:50660; flavin adenine dinucleotide binding
      GO:20037; heme binding
      GO:72341; modified amino acid binding
      GO:166; nucleotide binding
      GO:19826; oxygen sensor activity
      GO:5515; protein binding
      GO:16175; superoxide-generating NADPH oxidase activity
      Show all GO terms

      Homologs of NOX4 in Model Organisms

      Mus musculus
      Nox4
      Rattus norvegicus
      Nox4

      External links

      OMIM
      605261
      Ensembl
      ENSG00000086991
      Entrez Gene
      50507
      UniGene
      371036
      1000 Genomes
      1000 Genomes
      HPRD
      GenAtlas
      NOX4
      GeneCards
      NOX4