Cell Senescence Entries for NOX4
- Cell Types
- Breast epithelial, Lung fibroblast
- Cell Lines
- MCF-12A, TIG-3
- Cancer Cell?
- No
- Method
- Knockdown, Overexpression
- Type of senescence
- Replicative
- Senescence Effect
- Promotes
- Observations
- Stable overexpression of NOX4 in normal breast epithelial cells resulted in H2O2 production, cellular senescence, resistance to apoptosis, and tumorigenic transformation. In contrast, inhibition of Nox4 in lung fibroblasts by RNA interference blocked the RasV12 senescent phenotype including beta-galactosidase activity, growth arrest and accumulation of tumor suppressors such as p53 and p16. This suggests that Nox-generated ROS transduce senescence signals by activating the p53 and p16 pathways.
- Primary Reference
- Cell Types
- Breast epithelial, Lung fibroblast
- Cell Lines
- MCF-12A, TIG-3
- Cancer Cell?
- No
- Method
- Knockdown, Overexpression
- Type of senescence
- Replicative
- Senescence Effect
- Promotes
- Observations
- Stable overexpression of NOX4 in normal breast epithelial cells resulted in H2O2 production, cellular senescence, resistance to apoptosis, and tumorigenic transformation. In contrast, inhibition of Nox4 in lung fibroblasts by RNA interference blocked the RasV12 senescent phenotype including beta-galactosidase activity, growth arrest and accumulation of tumor suppressors such as p53 and p16. This suggests that Nox-generated ROS transduce senescence signals by activating the p53 and p16 pathways.
- Primary Reference
NOX4 Gene Information
- HGNC symbol
- NOX4
- Aliases
- KOX; KOX-1; RENOX
- Common name
- NADPH oxidase 4
- Entrez Id
- 50507
- Description
- This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]
NOX4 Ontologies
- Gene Ontology
-
Process: GO:45453; bone resorption
GO:55007; cardiac muscle cell differentiation
GO:7569; cell aging
GO:902; cell morphogenesis
GO:71320; cellular response to cAMP
GO:71480; cellular response to gamma radiation
GO:71333; cellular response to glucose stimulus
GO:71560; cellular response to transforming growth factor beta stimulus
GO:10467; gene expression
GO:50667; homocysteine metabolic process
GO:6954; inflammatory response
And 16 more GO terms Cellular component: GO:43020; NADPH oxidase complex
GO:16324; apical plasma membrane
GO:5789; endoplasmic reticulum membrane
GO:5925; focal adhesion
GO:16021; integral component of membrane
GO:5739; mitochondrion
GO:5730; nucleolus
GO:5634; nucleus
GO:48471; perinuclear region of cytoplasm
GO:1725; stress fiber
Function: GO:16174; NAD(P)H oxidase activity
GO:9055; electron carrier activity
GO:50660; flavin adenine dinucleotide binding
GO:20037; heme binding
GO:72341; modified amino acid binding
GO:166; nucleotide binding
GO:19826; oxygen sensor activity
GO:5515; protein binding
GO:16175; superoxide-generating NADPH oxidase activity
Homologs of NOX4 in Model Organisms
External links
- OMIM
- 605261
- Ensembl
- ENSG00000086991
- Entrez Gene
- 50507
- UniGene
- 371036
- 1000 Genomes
- 1000 Genomes
- HPRD
- GenAtlas
- NOX4
- GeneCards
- NOX4