Cell Senescence Entries for MDH1

Cell Types
Dermal fibroblast, Lung fibroblast
Cell Lines
IMR-90, Primary cell
Cancer Cell?
No
Method
Knockdown
Type of senescence
Replicative
Senescence Effect
Inhibits
Observations
In young dermal or lung fibroblasts knockdown of MDH1, which has reduced activity in old dermal fibroblasts, resulted in the appearance of significant cellular senescence features including SA-beta-galactosidase staining, flattened and enlarged morphology, increased population doubling time and elevated p16 and p21 protein levels.
Primary Reference

    MDH1 Gene Information

    HGNC symbol
    MDH1 
    Aliases
    MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375 
    Common name
    malate dehydrogenase 1 
    Entrez Id
    4190
    Description
    This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Feb 2016]

    MDH1 Ontologies

    Gene Ontology
    Process: GO:6734; NADH metabolic process
    GO:6094; gluconeogenesis
    GO:6108; malate metabolic process
    GO:6107; oxaloacetate metabolic process
    GO:6099; tricarboxylic acid cycle
    Cellular component: GO:5829; cytosol
    GO:70062; extracellular exosome
    GO:5615; extracellular space
    GO:5739; mitochondrion
    GO:43209; myelin sheath
    Function: GO:30060; L-malate dehydrogenase activity
    GO:51287; NAD binding
    GO:47860; diiodophenylpyruvate reductase activity
    GO:4470; malic enzyme activity
    GO:5515; protein binding
    Show all GO terms

    Homologs of MDH1 in Model Organisms

    Danio rerio
    mdh1a
    Drosophila melanogaster
    Mdh1
    Mus musculus
    Mdh1
    Rattus norvegicus
    Mdh1

    External links

    OMIM
    154200
    Ensembl
    ENSG00000014641
    Entrez Gene
    4190
    UniGene
    526521
    1000 Genomes
    1000 Genomes
    HPRD
    GenAtlas
    MDH1
    GeneCards
    MDH1