Cell Senescence Entries for GAPDH

Cell Types
Lung cancer
Cell Lines
A549
Cancer Cell?
Yes
Method
Knockdown
Type of senescence
Replicative
Senescence Effect
Inhibits
Observations
Knockdown of GAPDH in lung cancer cells (A549) resulted in a senescence phenotype, including proliferation arrest, morphological changes, increased SA-beta-galactosidase activity, and greater than 2-fold up-regulation of senescence-associated genes DEC1 and GLB1.
Primary Reference

    GAPDH Gene Information

    HGNC symbol
    GAPDH 
    Aliases
    G3PD; GAPD; HEL-S-162eP 
    Common name
    glyceraldehyde-3-phosphate dehydrogenase 
    Entrez Id
    2597
    Description
    This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

    GAPDH Ontologies

    Gene Ontology
    Process: GO:61621; canonical glycolysis
    GO:71346; cellular response to interferon-gamma
    GO:6094; gluconeogenesis
    GO:226; microtubule cytoskeleton organization
    GO:17148; negative regulation of translation
    GO:51402; neuron apoptotic process
    GO:35606; peptidyl-cysteine S-trans-nitrosylation
    GO:50821; protein stabilization
    GO:16241; regulation of macroautophagy
    Cellular component: GO:97452; GAIT complex
    GO:5737; cytoplasm
    GO:5829; cytosol
    GO:70062; extracellular exosome
    GO:31012; extracellular matrix
    GO:43231; intracellular membrane-bounded organelle
    GO:30529; intracellular ribonucleoprotein complex
    GO:5811; lipid particle
    GO:16020; membrane
    GO:15630; microtubule cytoskeleton
    GO:31965; nuclear membrane
    And 5 more GO terms
    Function: GO:51287; NAD binding
    GO:50661; NADP binding
    GO:97718; disordered domain specific binding
    GO:4365; glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
    GO:42802; identical protein binding
    GO:8017; microtubule binding
    GO:35605; peptidyl-cysteine S-nitrosylase activity
    GO:5515; protein binding
    Show all GO terms

    Homologs of GAPDH in Model Organisms

    Caenorhabditis elegans
    gpd-4
    Danio rerio
    gapdh
    Drosophila melanogaster
    Gapdh2
    Mus musculus
    Gm6316
    Rattus norvegicus
    RGD1562758
    Schizosaccharomyces pombe
    tdh1

    External links

    OMIM
    138400
    Ensembl
    ENSG00000111640
    Entrez Gene
    2597
    UniGene
    544577
    1000 Genomes
    1000 Genomes
    HPRD
    GenAtlas
    GAPDH
    GeneCards
    GAPDH