Cell Senescence Entries for DGCR8

Cell Types
Lung fibroblast
Cell Lines
IMR-90
Cancer Cell?
No
Method
Knockdown
Type of senescence
Replicative
Senescence Effect
Inhibits
Observations
The impact of global disruption of miRNA biogenesis in primary fibroblasts was investigated through the knockdown of DGCR8, an essential mediator of the synthesis of canonical miRNAs. The inactivation of DGCR8 resulted in a dramatic antiproliferative response, with the acquisition of a senescent phenotype. Senescence triggered by DGCR8 loss was accompanied by the upregulation of the cell-cycle inhibitor p21.
Primary Reference

    DGCR8 Gene Information

    HGNC symbol
    DGCR8 
    Aliases
    Gy1; pasha; DGCRK6; C22orf12 
    Common name
    DGCR8, microprocessor complex subunit 
    Entrez Id
    54487
    Description
    This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

    DGCR8 Ontologies

    Gene Ontology
    Process: GO:90502; RNA phosphodiester bond hydrolysis, endonucleolytic
    GO:31047; gene silencing by RNA
    GO:31053; primary miRNA processing
    GO:72091; regulation of stem cell proliferation
    Cellular component: GO:5737; cytoplasm
    GO:70877; microprocessor complex
    GO:5730; nucleolus
    GO:5654; nucleoplasm
    GO:5634; nucleus
    Function: GO:3725; double-stranded RNA binding
    GO:20037; heme binding
    GO:46872; metal ion binding
    GO:70878; primary miRNA binding
    GO:5515; protein binding
    GO:42803; protein homodimerization activity
    GO:4525; ribonuclease III activity
    Show all GO terms

    Homologs of DGCR8 in Model Organisms

    Caenorhabditis elegans
    pash-1
    Danio rerio
    dgcr8
    Drosophila melanogaster
    pasha
    Mus musculus
    Dgcr8
    Rattus norvegicus
    Dgcr8

    External links

    OMIM
    609030
    Ensembl
    ENSG00000128191
    Entrez Gene
    54487
    UniGene
    643452
    1000 Genomes
    1000 Genomes
    HPRD
    GenAtlas
    DGCR8
    GeneCards
    DGCR8