Cell Senescence Entries for AAK1

Cell Types
Lung fibroblast, Embryonic kidney
Cell Lines
IMR-90, MRC-5, HEK293T
Cancer Cell?
No
Method
Overexpression
Type of senescence
Replicative
Senescence Effect
Promotes
Observations
A library of 200 constitutively activated kinases was screened in order to identify kinases whose constitutive expression decreased cell proliferation and induced expression of senescence markers; p16 and SASP components. A total of 33 kinases showed pro-senescence effects (particularly MAP3K7, PRKCD, and MATK); kinases with strong pro-senescence effects, induced expression of SASP-component genes (e.g. IL1A, IL1B, IL6 and IL8) through activation of an NF-kappaB-dependent transcriptional program. Inhibition of the NF-kappaB, p53, or Rb pathway proved insufficient to prevent kinase-triggered cell cycle arrest.
Primary Reference

    AAK1 Gene Information

    HGNC symbol
    AAK1 
    Aliases
     
    Common name
    AP2 associated kinase 1 
    Entrez Id
    22848
    Description
    Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

    AAK1 Ontologies

    Gene Ontology
    Process: GO:6897; endocytosis
    GO:61024; membrane organization
    GO:45747; positive regulation of Notch signaling pathway
    GO:46777; protein autophosphorylation
    GO:6468; protein phosphorylation
    GO:50821; protein stabilization
    GO:2000369; regulation of clathrin-dependent endocytosis
    GO:32880; regulation of protein localization
    Cellular component: GO:31252; cell leading edge
    GO:5905; clathrin-coated pit
    GO:30136; clathrin-coated vesicle
    GO:5737; cytoplasm
    GO:5829; cytosol
    GO:19897; extrinsic component of plasma membrane
    GO:43195; terminal bouton
    Function: GO:35612; AP-2 adaptor complex binding
    GO:5524; ATP binding
    GO:5112; Notch binding
    GO:5515; protein binding
    GO:4674; protein serine/threonine kinase activity
    Show all GO terms

    Homologs of AAK1 in Model Organisms

    Caenorhabditis elegans
    sel-5
    Danio rerio
    aak1
    Drosophila melanogaster
    Nak
    Mus musculus
    Aak1
    Rattus norvegicus
    Aak1

    In other databases

    GenAge model organism genes
    • A homolog of this gene for Caenorhabditis elegans is present as sel-5

    External links

    OMIM
    616405
    Ensembl
    ENSG00000115977
    Entrez Gene
    22848
    UniGene
    468878
    1000 Genomes
    1000 Genomes
    HPRD
    GenAtlas
    AAK1
    GeneCards
    AAK1